Interrogating Androgen Receptor Mediated Gene Expression and Tumor Progression by Molecular Imaging
Final rept. 9 Sep 2002-8 Sep 2005
CALIFORNIA UNIV LOS ANGELES
Pagination or Media Count:
This final report summarizes our work on the effectiveness of a new adenovirus based optical imaging vector designed to detect MAP kinase levels in living subjects. The vector was injected into human tumors implanted into SCID mice. Animals were injected with D-luciferin and imaged in a Xenogen IVIS device. The vector was able to detect enhanced MAPK levels stimulated by Epidermal Growth Factor in prostate cancer tumors of xenograft animals. We interrogated two lines of androgen dependent AD and independent Al xenograft animal models CWR22 and LAPC9 to determine MAPK levels. We found no significant difference between AD and Al cancer with respect to functional MAPK levels. However, CWR22 animals exhibit significantly higher MAPK levels than LAPC9 xenograft animals. The data suggest the possibility that gene expression based imaging may be able to detect augmented MAPK levels predictive of disease progression as well as the efficacy of cancer therapeutics.
- Medicine and Medical Research