Biofluidic Intelligent Processors for Preparative Manipulations of Biological Warfare Agents at the Attomole Level
Final rept. Jun 2000-Sep 2004
ILLINOIS UNIV AT URBANA BECKMAN INST
Pagination or Media Count:
A new type of biofluidic transport system, the Biofluidic Intelligent Processor BIP, was developed which specifically used biological compounds to adaptively control the transport of microfluids. The multicompartment, multimembrane BIP system was designed, fabricated and tested. The BIP had characteristic linear dimensions of nanometers and volumes as small as tens of attoliters, and was specifically designed to manipulate nano-amounts of species. Electrokinetic and other standard microfluidic flows were exploited to move species among the separate compartments of the system, where molecules were selectively separated and transported from the influent to the detector, independent of the other constituents within the biofluid. Within the new biofluidic BIP system were integrated compartments, including molecular gates, which were based on novel membranes that performed important biomolecular manipulations that included affinity binding and molecular sieving. The molecular gate concept is analogous to transistors, in that switching, gain, and digital manipulation of specific molecules, such as proteins, are now possible.
- Chemical, Biological and Radiological Warfare