Smart Gold Nanobowls (Nano-Crescent Moon) with Sub-10 nm Circular Edge for Local Electromagnetic Field Enhancement Effect, Spatial, and NIR Temporal/Thermal Modulations for Molecular and Cellular Dynamic Imaging
Final rept., 1 Jun-31 Oct 2005
CALIFORNIA UNIV BERKELEY
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Cell signaling involves the production and activation of many intracellular biomolecules. The dynamic detection of the biomolecular reactions is essential to understand the undefined signaling pathway. Current fluorescence based methods have difficulty detecting the dynamic reactions of biomolecules such as nucleolytic, proteolytic, and phosphorylation. In contrast, label-free Raman scattering spectroscopy can detect subtle biochemical changes without fluorescent molecules. First, we have created gold-based nanocrescent Surface Enhanced Raman Scattering SERS probes which can function as the substrate to enhance Raman scattering signal for tens of orders of magnitude. Once functionalized with oligonucleotides and peptides, the internalized nanocrescent particles can be used to monitor the enzymes inside the single living cell. In the past ten months, in addition to gold-based nanocrescent SERS probes, we have accomplished the multilayer composite nanocrescent SERS probes with magnetic thin film layer inside of gold nanocresents.
- Atomic and Molecular Physics and Spectroscopy