Targeting Microvascular Pericytes in Angiogenic Vessels of Prostate Cancer
Annual rept. 1 Apr 2004-31 Mar 2005
LA JOLLA INST FOR MOLECULAR MEDICINE SAN DIEGO CA
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Pericytes are critical for neovascularization to form and maintain angiogenic sprouts in cancer. In May 2005 annual report we elucidate an extrinsic pharmacological targeting method, consisting of loading hydron polymer pellets with anti-NG2 neutralizing antibody to target nascent pericytes in the neovasculature of PC3 and LNCaP tumors. Hydron pellets containing NG2 neutralizing antibody decreased corneal neovascularization induced by PC3 tumor xenografts significantly. Mean corneal neovascularization in the treated eyes and control eyes were 0.5341 mm2, and 2.789mm2 respectively n20 eyes, pO.0001. Hydron pellets containing NG2 neutralizing antibody decreased corneal neovascularization induced by LNCaP tumor xenografts significantly. Mean corneal neovascularization in the treated eyes and control eyes were 0.3393 mm2, and 3.443 mm2 respectively nl0 eyes, p-0.0079. We have also encountered extensive lymphangiogenesis in PC3, LNCaP and TRAMP tumors in addition to angiogenesis. We have grown TRAMP prostate tumors in NG2 knockout and wild type mice to elucidate the anti-neovascular effect of intrinsic genetic targeting of NG2 on nascent pericytes. Task 3B of our statement of work is under way We are in the process of comparing these tumor tissues by using an image analysis software Volocity in terms of microvascular density and lymphatic endothelial density.
- Medicine and Medical Research