Analysis of Genes Differentially Expressed in a Human Ovarian Cancer Model
Final rept. 1 Jul 2001-30 Jun 2005
WAYNE STATE UNIV DETROIT MI
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The objectives of this study were to 1 analyze gene expression in a model of human ovarian cancer from a benign to a malignant phenotype in cells grown in organotypic culture and 20 confirm gene expression patterns by RNA analysis. Two human cell lines were used, 96.9.18 and 1.24.96, and RNA extracted from cells at various stages of malignant change were queried on Affymetrix HU133A and B chips in triplicate 96.9.18 or HU133A plus chip in triplicate 1.24.96 . Data analysis has revealed a number of genes that are dysregulated in both 96.9.18 and 1.24.96. An analysis of the interaction of these genes showed that 5 genes are downregulated between the two cell lines and 183 genes are upregulated during malignant progression. Downregulated genes are too few to analyze but the upregulated genes are involved in apotosis, cytokine-cytokine receptor interactions, toll-like receptor signaling pathway, and the MAPK signaling pathway.
- Anatomy and Physiology
- Medicine and Medical Research