Naked DNA Immunization for Prevention of Prostate Cancer in a Dunning Rat Prostate Tumor Model
Annual rept. 1 Jun 2004-31 May 2005
GEORGE WASHINGTON UNIV WASHINGTON DC
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In year three of the award period, we tested the effects of pre-immunization of rats against PSNA, PSA or PAP on the development of transplantable tumors. Immunization of rats with a cocktail consisting of plasmid DNA and rat GN-CSF against PAP, PSA or PSNA protected the animals against development of transplantable AT3-P1 PSA tumors. None of the animals immunized with H PSA-T or H PSNA-T developed antibodies against the target antigen. In contrast, immunization with the secreted vaccines, HPSNA-S or HPSA-S resulted in production of antibodies against the target antigen. The antibodies were of mixed Th1 and Th2 type IgG1 and IgG2a. When priming was performed with the truncated version of the vaccines H PSNA-T or H PSA-T, however and boosting with the secreted ones, the antibodies were mainly of the Th1 complement-binding type IgG2a and IgG2b . The best protection against PSNA-transfected AT3-P1 tumors was achieved when priming was performed with a plasmid encoding a xenogeneic protein and boosting with a plasmid encoding a syngeneic one.
- Anatomy and Physiology