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Arginase: A Novel Proliferative Determinant in Prostate Cancer

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Annual rept. 1 Apr 2004-31 Mar 2005

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This project is an investigation of the involvement of the enzyme arginase type II All in the pathogenesis and growth of prostate cancer. Having cloned the All gene in our laboratory, we unexpectedly discovered that it is expressed at high levels in the normal prostate and even higher in neoplastic prostate samples. The purpose of the present research funded by USAMRMC is to examine the expression of All in a wider range of benign and- malignant prostate specimens and cultured cells to determine its usefulness as a novel marker of prostatic neoplasia and the extent of its involvement in cancer pathogenesis We are also exploring whether specific chemical and molecular inhibitors of arginase and several related enzymes in the polyamine metabolic pathway might suppress or arrest the growth of prostate cancer cells in vitro or in vivo. This second annual report describes our progress over the past year in characterizing the overexpression and inhibitory vector constructs used for these experiments, our optimization of small inhibitory RNA transfer techniques in prostatic cancer cells, the construction and study of stable arginase- overexpressing prostatic cell lines, the correlation of polyamine synthesis levels in these lines, and our measurement and localization of arginase expression in benign and malignant prostate tissue samples.

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  • Anatomy and Physiology
  • Medicine and Medical Research
  • Organic Chemistry

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