Optical Constant Determination of Bacterial Spores in the MIR
Final rept. 1 Jul 2004-31 Mar 2005
SOUTH CAROLINA UNIV COLUMBIA OFFICE OF SPONSORED PROGRAMS
Pagination or Media Count:
We developed a simple method for estimating the real and imaginary refractive index of particles. This is done by filtering the particles bacterial spores in our case on a gold-coated filter membrane and performing IR microspectroscopy. The results are extremely reproducible within particle counting statistics. A Kramers-Kronig consist iterative algorithm is applied to obtain scattering and absorption cross-sections for individual particles. The method was tested on spores and on polystyrene spheres.