Prostatic Fluid Cells
Final rept. 1 Apr 2001-30 Apr 2005
CASE WESTERN RESERVE UNIV CLEVELAND OH
Pagination or Media Count:
Most research that requires long-term propagation of prostate cancer PCA cells is carried out with three cell lines DU 145, PC-3, and LNCaP. All but one of these lines, LNCaP, fail to express prostate specific antigen PSA, androgen receptors, andor any other prostate phenotype. Some prostatic fluids contain PCA cells. Our goals were 1 to test the tumorigenicity of and to develop transplantable xenografts from POA cells in prostatic fluid, 2 to develop methods for enhancing the tumorigenicity of small numbers of these PCA cells without deliberately altering their genes, 3 to test these methods for enhancement of tumorigenicity with prostatic fluid cells, and 4 to initiate clinical follow-up. As detailed in the body of our report, our aims that required prostatic fluid have been limited by exceptionally weak clinical collaboration that has become progressively weaker. The fluids that we tested earlier werepromising. The quantity and quality of prostatic fluids received from our clinical Collaborator, Dr. Martin Resnick, declined progressively. Coinjection of lethally irradiated, growth-factor-producing cells was encouraging in some experiments in the case of some tumors however, results were quite variable in repeated experiments.
- Anatomy and Physiology
- Medicine and Medical Research