Accession Number:

ADA439222

Title:

Mechanism of Hormonal Regulation of CAD Gene Expression in Human Breast Cancer Cells

Descriptive Note:

Annual summary

Corporate Author:

TEXAS A AND M RESEARCH FOUNDATION COLLEGE STATION

Personal Author(s):

Report Date:

2005-05-01

Pagination or Media Count:

21.0

Abstract:

The CAD gene is trifunctional and expresses carbamoylphosphate synthetaseaspattate casbamyltransferaseditiydoorotase, wbich are required for pyrimidine biosynthesis. TCDD inhibited E2-induced expression of CAD gene and also downregulated E2-mediated transactivation of CAD gene promoter constructs in transient transfection assays. Moreover the CAD gene promoter construct with a mutant DRE was also inhibited by TCDD suggesting that DRE is not important in Ahr mediated inhibition of CAD gene expression. Using fluorescence resonance energy transfer FRET, we show that TCDD inhibits the direct interaction between ERa and Spi that is observed in presence of E2.

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE