Accession Number:

ADA439213

Title:

Identification of Functions of Neurofibromin Distinct From the RasGAP Domain

Descriptive Note:

Annual rept. 1 May 2004-30 Apr 2005

Corporate Author:

CALIFORNIA UNIV SAN FRANCISCO

Report Date:

2005-05-01

Pagination or Media Count:

9.0

Abstract:

We proposed to study new functions of neurofibromin in the model organism, the budding yeast, Saccharomyces cerevisiae. The design of our study is mainly to generate dominant negative mutants of neurofibromin and its yeast homologs Ira1 and Ira2, induce their. expression in wild type yeast and elicit an interfering phenotype. We will then screen a yeast library to identify novel interaction partners of neurofibromin. We have constructed various mutants and fragments of Iral and neurofibromin. All of them have been tested in either transient or stably integrated expression. So far we have not observed any obvious interfering phenotype in the yeast strains that we have used. We have also experienced difficulties in detecting the expression of some of the mutants. We are continuing to test more yeast strains as difference strains have variable sensitivities to these mutants. A good rabbit anti-neurofibromin antibody was generated. Our second methodology to search for new functions of neurofibromin is gene expression profiling. We have obtained some preliminary data comparing IralIra2 deletion and Ras super-activation. We are working towards verifying these data and hopefully reaching some definitive answers in the near future.

Subject Categories:

  • Biochemistry
  • Genetic Engineering and Molecular Biology
  • Biology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE