Vasculature-Specific Adenovirus Vectors for Gene Therapy of Prostate Cancer
Annual rept. 1 Feb 2004-31 Jan 2005
M D ANDERSON CANCER CENTER HOUSTON TX
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In Year 1 of the project we have completed Task 1 as per approved Statement of Work by generating a panel of plasmid vectors for the expression of a series of modified adenovirus fiber genes. These genes encode genetic fusions of the adenovirus fiber protein, which has been stripped off its native receptor binding site by mutagenesis, with the previously identified peptides that are specific for the prostate tumor vasculature. The resultant proteins have been transiently expressed in cultured human cells and characterized by Western blot analysis. As a result of this work, we have identified those fiber-peptide fusion proteins that retain the trimeric structure of native adenovirus fiber protein and, thus, can be incorporated into the capsid of Ad vector for tumor targeting.
- Genetic Engineering and Molecular Biology
- Medicine and Medical Research
- Anatomy and Physiology