Accession Number:

ADA434636

Title:

Quest: A New Approach to Molecular Staging of Tumors

Descriptive Note:

Final rept. 30 Jul 2001-29 Jul 2004

Corporate Author:

WASHINGTON UNIV SEATTLE

Personal Author(s):

Report Date:

2004-08-01

Pagination or Media Count:

54.0

Abstract:

Lack of a definitive system for the diagnosis and staging of NF1-related tumors is a major obstacle to investigating the molecular basis of tumorigenesis, to our ability to assess prognosis, and consequently, to the rational design and application of stage-specific therapeutic agents 1-3. Locus-specific changes in copy number are a common feature of many types of tumors, including the neurofibromas and malignant peripheral nerve sheath MPNST that develop in individuals with NF1 4-13. Gains or losses at a particular locus are reviewed in 4 potential biomarkers for the molecular diagnosis and grading of MPNST. Towards the goal of molecular diagnosis and staging of NF1-related tumors, we propose to develop and validate a novel polymerase chain reaction PCR-based method, termed QuEST, for rapid and quantitative identification of loci with increases or decreases in genomic copy number in MPNST.

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE