Searching the Epigenome for Novel Breast Cancer Tumor Suppressor
Annual rept. 1 Sep 2002-31 Aug 2003
COLD SPRING HARBOR LAB NY
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The initial proposal focused on developing technologies to uncover epigenetic changes that contribute to tumor development. The authors initial attempts towards developing genome-wide approaches to identifying new genes silenced by epigenetic mechanisms encountered problems. However, their efforts to exploit epigenetic mechanisms of gene silencing to study tumor suppressor gene function have been very successful. Therefore, as they enter the second year of funding, they plan to capitalize on the success of the latter experiments in refocusing their efforts. Their new objectives will build upon the unanticipated advances that they have made in the use of RNAi to manipulate gene expression in mouse and human cell systems and in animal models to explore the role of epigenetic modifications in breast cancer progression. This report presents key accomplishments in the following areas control of cellular senescence and the silencing of E2F target genes, analysis of the CBX7 oncogene, use of ribonucleic acid interference RNAI to suppress gene expression and produce tumor phenotypes in mice, and RNAI libraries and other new tools.
- Genetic Engineering and Molecular Biology
- Medicine and Medical Research