Regulation of Cdc42/Rac Signaling in the Establishment of Cell Polarity and Control of Cell Motility
Annual summary rept. 1 Aug 2001-31 Jul 2004
DUKE UNIV MEDICAL CENTER DURHAM NC
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Cdc42p, together with other polarity proteins, becomes polarize to a cap at the presumptive bud site and the tip of the emerging bud. The initial observation that Cdc42p is able to polarize, and remain polarized, in the complete absence of F-actin Ayscough et al., 1997, has been confirmed repeatedly by many laboratories including ours. These studies use Lat-A as a quick, effective method to completely depolymerize actin, and led to the conclusion that Cdc42p polarization is actin-independent. Polarized secretion and endocytic uptake require F-actin cables and patches, respectively. When Lat-A is applied to yeast cells, neither actin patches nor cables are detectable, and all F-actin-dependent processes are disrupted. Thus, Cdc42p polarization occurs by a non-secretory pathway.
- Anatomy and Physiology
- Medicine and Medical Research