Accession Number:

ADA433948

Title:

Biomarkers of Selenium Action in Prostate Cancer

Descriptive Note:

Annual summary rept. 1 Jan-31 Dec 2004

Corporate Author:

STANFORD UNIV CA

Personal Author(s):

Report Date:

2005-01-01

Pagination or Media Count:

42.0

Abstract:

This study was designed to identify new, mechanistically relevant biomarkers of selenium responsiveness for use in intervention trials. We have completed our first aim which is to identify common molecular targets of different forms of selenium that are secretory using bioinformatics approaches and datasets of selenium-modulated transcripts and membrane bound and secretory proteins. 17 genes and 123 named unique genes that are membrane boundsecretory and upregulated by three or two forms of selenium respectively were selected. Out of these 140 genes, 15 were shown to be secretory by conventional methods according to published literature. In addition, we have determined the similarities and differences in global gene expression charges induced by methylseleninic acid MSA and selenomethionine SM in prostate cancer cells LNCaP. 2,336 and 2,165 unique genes showed expression changes at least 1.5 fold in at least 3 samples in response to SM and MSA respectively. 366 genes were statistically significantly differentially expressed between MSA and SM treated cells. At the same concentration, SM and MSA modulated press. However, they exert their effects on LACaP cells through both different and common molecular targets.

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE