Characterization of the Role of JJAZ1 in Human Breast Cancer
Final rept. 1 Jul 2003-31 Dec 2004
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We hypothesized that the upregulation of the PcG protein JJAZ1 now known as Suz12 in human breast cancers leads to chromatin modification and subsequent changes in gene expression. It is difficult to identify genes regulated by PcG proteins due to the fact that these proteins do not directly bind to DNA, but rather are recruited via one or more site-specific DNA binding proteins. Our overall Aim was to demonstrate that our recently developed method, which combines chromatin immunoprecipitation and promoter microarray analysis, could be used to identify a large set of genes that are regulated by JJAZ1Suz12. We proposed to develop an antibody to JJAZ1Suz12 and then to use that antibody in combination with a technique which we have developed called ChlP-CpG that would allow us to identify the chromosomal sites bound by JJAZlSuz12 without prior knowledge of its DNA-binding protein partners. We were successful in developing an antibody to Suz12, have demonstrated that the ChlP-CpG assay can identify Suz12 targets in colon cancers, have collected a number of human breast cancer samples, and are currently completing the studies to identify breast cancer-specific JJAZ1Suz12 target genes.
- Medicine and Medical Research