Identification of Aedes aegypti and its Respective Life Stages by Real-Time PCR
OFFICE OF THE SURGEON GENERAL (AIR FORCE) WASHINGTON DC
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An Aedes aegypti specific fluorogenic probe hydrolysis TaqMan PCR assay was developed for real-time screening using a field-deployable thermocycler. Laboratory-based testing of Ae. aegypti, Ae. aegypti Trinidad strain Culex pipiens Culex pipiens quinquefasciatus Anopheles stephensi Ochlerotatus taeniorhynchus individual adult mosquitoes and mixed pools n10 demonstrated 100 concordance in both in vitro sensitivity 66 and specificity 1010. A single adult Aedes aegypti was identified in a pool of 100 non-Aedes aegypti mosquitoes. The limit of detection of Aedes aegypti egg pools was 5 individual eggs. Field-testing was conducted in central Honduras. An Aedes aegypti and Culex spp. panel of individual and mixed pools n 30 of adult mosquitoes, pupae, and larvae demonstrated 100 concordance in sensitivity 2222 and 97 concordance in specificity 2930 with one false positive. Field-testing of an Aedes aegypti and Culex spp. blind panel n 16 consisting of individual and mixed pools of adult mosquitoes, pupae, and larvae demonstrated 90 concordance in sensitivity 910 and 88 concordance in specificity 1416.
- Medicine and Medical Research