Biofluidic Transport and Molecular Recognition in Polymer Microdevices
Final rept. Feb 2002-Jan 2005
COLORADO UNIV AT BOULDER
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Polymeric microfluidic components were fabricated using living-radical photopolymerization LRPP, including three-dimensional geometries, modified surfaces by grafting, microporous mixersfilters, micropumps, wires, and movable parts. LRPP enables controlled geometry and surface chemistry for fluid control, biomolecular and cell detection, and particle sorting. Key accomplishments include specific cell adhesion and cytocompatibility demonstrated with grafted surfaces, a fluid-responsive polymer micropump integrated on a device and characterized, a porous polymer plug fabricated within a microfluidic channel and used to mix adjacent streams, analyses and verification of wall effects on particle motion in narrow channels, a polymerizable resistance heater as an electrolysis pump, cell detection with fluorescence when cells adhere to a modified surface, rapid antigenantibody detection at 1 pM, hybrid siliconpolymer microfluidic devices, flexible membranes and septums for pumping and sample injection, and development of microfluidic devices with arrays of wells and cells.
- Physical Chemistry