Accession Number:

ADA433098

Title:

Genetic and Functional Studies of Genes that Regulate DNA-Damage-Induced Cell Death

Descriptive Note:

Annual rept. 1 May 2003-31 Oct 2004

Corporate Author:

BAYLOR COLL OF MEDICINE HOUSTON TX

Personal Author(s):

Report Date:

2004-11-01

Pagination or Media Count:

7.0

Abstract:

Studies have shown that apoptosis and survival pathways in response to DNA damage play a critical role in breast cancer development and progression. 90 of breast cancer cases are sporadic where mutations of BRCA12 have not been detected. Other breast cancer genes must exist. Our group has approached the issue in two ways, a genomic and a proteomic approach. We have established and utilized a novel retrovirus-based genetic screen system to search for genes that would confer resistance to DNA damage induced apoptosis. Multiple clones have been isolated from this genetic screen. Among the genes identified are both novel and known proteins that may be important in DNA-damage responses. To further elucidate the pathways mediated by BARD1, we looked for factors interacting with BARD1 using mass spec sequencing. Several factors have emerged from this study and are being examined. The information obtained from our studies should prove useful for developing new and effective screening strategies, drug targets, and treatment for breast cancer.

Subject Categories:

  • Genetic Engineering and Molecular Biology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE