Accession Number:

ADA433013

Title:

Raman and Surface Enhanced Raman of Biological Material

Descriptive Note:

Conference paper

Corporate Author:

EDGEWOOD CHEMICAL BIOLOGICAL CENTER ABERDEEN PROVING GROUND MD

Report Date:

2004-12-01

Pagination or Media Count:

6.0

Abstract:

In light of recent world events, an emphasis has been placed firmly on the development of rapid biological threat detection techniques for the military as well as for homeland security. Vibrational spectroscopic methods such as Raman and surface enhanced Raman scattering SERS provide rapid detailed fingerprint information about the molecular composition of biomaterial in a non-destructive manner. The technology and methodology to acquire and interpret biological Raman spectra has seen tremendous advances over the past several decades. The problem of tissue fluorescence, which overwhelms the Raman signal of most biological samples upon excitation in the visible region, has been largely overcome by instrumentation operating in the near-IR region of the spectrum. In addition, treatment of biomaterials with silver nanoparticles not only enhances the normal Raman signal by several orders of magnitude but also further reduces the fluorescence backgrounds via interaction between the analyte and the metal SERS substrate. This work presents a study into the applicability of qualitative Raman spectroscopy with principal component analysis PCA and surface enhanced Raman for quantitative analysis of the biological threat materials associated with the Rapid Agent Aerosol Detection RAAD project.

Subject Categories:

  • Atomic and Molecular Physics and Spectroscopy
  • Chemical, Biological and Radiological Warfare
  • Miscellaneous Detection and Detectors

Distribution Statement:

APPROVED FOR PUBLIC RELEASE