Mammary Stem Cell Isolation
Final rept. 21 Jul 2003-20 Jul 2004
MARYLAND UNIV BALTIMORE
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The objective of this research was to identify mouse mammary gland stem cells, with the ultimate goal being their isolation. We hypothesized that mammary gland stem cells can be identified by generating transgenic mice using a LEFTCF-dependent reporter gene as a marker. Furthermore, by using green fluorescent protein GFP as the reporter, putative stem cells could be isolated by fluorescence activated cell sorting. The Specific Aims of the proposal were 1 Generate transgenic mouse lines expressing GFP from a LEFTCF- dependent promoter 2 Determine sites of GFP transgene expression during mammary gland development, involution and remodeling. The ability to identify and isolate stem cells would simplify their characterization as well as the study of factors necessary for proliferation, differentiation, and susceptibility to transformation. Aim I was successfully completed, resulting in the generation of 4,Lef-GFP transgenic founders.
- Anatomy and Physiology
- Medicine and Medical Research