Bone Sialoproteins and Breast Cancer Detection
Annual rept. 1 Jul 2003-10 Jun 2004
JOHNS HOPKINS UNIV BALTIMORE MD
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We have been studying a family of proteins that we have termed SIBLINGs for Small Integrin Binding LIgand N-linked Glycoproteins, that share similar structural domains, human chromosomal location, normal synthesis by skeletal tissue, and abnormal expression by neoplasms. The goal of the our research is to test whether SIBLINGs might be informative markers for breast cancer detection. To accomplish this goal we have developed competitive enzyme-linked immunosorbent assays ELISAs for the SIBLINGs bone sialoprotein BSP, osteopontin OPN, dentin matrix protein-1 DMP1, dentin sialophosphoprotein DSPP and matrix extracellular phosphoglycoprotein MEPE . Sandwich-based ELISA assays have also been developed. When the competitive ELISAs were used to screen SIBLING protein levels, BSP and OPN exhibited the highest degree of sensitivity and specificity for the detection of breast cancer. When SIBLING levels in breast cancer patient-derived samples were segregated grossly by tumor type lobular versus ductal, statistically significant differences between the two groups were observed. Microarray analysis of normal and breast cancer-derived mRNA samples found a similar elevated levels of elevated SIBLING expression and differences by tumor type. These results suggest that SIBLINGs may have utility as serum-based markers for breast cancer detection.
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- Medicine and Medical Research