Observation of Cell-Material Interface at Cell Detachment
RECONSTITUTION MATERIALS GROUP TSUKUBA IBARAKI (JAPAN)
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A single cell adhering to glass surface was detached from material surface by applying shear for using a microcantilever. The interface between the cell and glass surface was observed by interference reflection microscopy during the cell detachment. As a result, it was found that focal adhesion proteins remained on glass surface after the detachment. Fibrous structures which may be stress fibers also remained attaching to the focal adhesion proteins. Immunofluoresccent staining confirmed that Beta1-integrins remained on the glass surface after the detachment. The facts suggest that cell-material adhesion is fractured inside the cell when the cell is detached by shear force.
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