Sprouty-1, an Inhibitor of Prostate Cancer Signal Transduction
Final rept. 1 Jul 2001-30 Jun 2004
BAYLOR COLL OF MEDICINE HOUSTON TX
Pagination or Media Count:
A considerable body of evidence indicates that alterations of fibroblast growth factors FGF and their receptors contribute to prostate cancer progression. Recently a new family of regulators of FGF activity has been identified. The Sprouty gene family negatively regulates FGF signaling in a variety of systems and could potentially limit the biological activity of FGFs in prostate cancer. Immunohistochemical analysis of normal and neoplastic prostate tissues using tissue microarrays revealed that Sprouty-1 protein is down-regulated in approximately 40 of prostate cancers when compared with matched normal prostate. By quantitative real-time PCR analysis we found that Sprouty-1 mRNA levels were significantly decreased in prostate cancers in vivo in comparison to normal prostate. In prostate cancer cell lines there is loss of the normal up-regulation of Sprouty-1 mRNA and protein in response to FGFs. The decrease in Sprouty-1 expression in the human prostate cancer, despite elevated levels of FGF ligands and FGF receptors, implies a loss of an important growth regulatory mechanism in prostate cancers that may potentiate the effects of increased FGF and FGF receptor expression in prostate cancer.
- Anatomy and Physiology
- Medicine and Medical Research