Improving Blood Monitoring of Enzymes as Biomarkers of Risk From Anticholinergic Pesticides and Chemical Warfare Agents
Annual rept. 24 Sep 2003-23 Sep 2004
CALIFORNIA UNIV DAVIS
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Blood biomarkers are an important way to monitor exposure to anticholinergic pesticides and chemical warfare agents and to establish whether some are at greater risk than others from exposure to them. Many clinical and research laboratories use the colorimetric Ellman assay based on the hydrolysis of acetylthiocholine. CHPPM US Army Center for Health Promotion and Preventive Medicine uses a slower delta pH method based on that of Michel to monitor 16,000 DOD personnel each year. This year OP exposure was mimicked by treating whole blood from UCD University of California, Davis with DFP, to give a range of AChE activity. RBC AChE activity was measured using both the Ellman and delta pH assays at UCD. An estimated conversion factor was calculated. A normal range of AChE activities from the CHPPM delta pH assay was converted to Ellman units. These ranges are important benchmarks for clinical laboratory ChE determinations in the absence of baseline data.
- Medicine and Medical Research
- Chemical, Biological and Radiological Warfare