Assessment of the Activation State of Rho Family GTP-Binding Proteins in Breast Cancer Cells and Specimens
Final rept. 3 Jul 2000-2 Jul 2004
CHILDREN'S HOSPITAL MEDICAL CENTER CINCINNATI OH
Pagination or Media Count:
Rho GTPases are known to play roles in various aspects of cancer development. To establish a causal link between activation of individual Rho GTPases and certain aspects of breast cancer, we have started to search for a functional association of Rho GTPases with mitogenic conditions and various physiological or pathological situations that may be connected to mammary cell transformation. As stated in this final report, we have established a method using the Rho GTPase-interactive effector domains of WASP, PAK 1, and Rhotekin to semi-quantitatively define the activation states of Rho family members Cdc42, Rac 1, and RhoA in cancer cells, and have applied this method to a number of cellular conditions which are related to mammary tumorigenesis. These studies are significant since the findings further confirmed the involvement of Rho GTPases in diverse physiological and pathological situations that may contribute to mammary carcinogenesis. The results obtained strongly support our long term goal to implicate specific Rho GTPases in breast cancer development and to devise novel anti-cancer therapeutics targeting individual Rho signaling pathways.
- Medicine and Medical Research