The Role of Claudin-7 in Mouse Mammary Gland and Tumorigenesis
Final rept. 1 Jul 2001-30 Jun 2004
COLORADO UNIV HEALTH SCIENCES CENTER AURORA CO
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We have fully characterized the expression and localization of claudin 7 in the mammary epithelium, finding that it is not associated with tight junctions but is constitutively present in punctate structures, presumably vesicles, at the basal and lateral borders of cells. The same is true of bronchiole and certain elements of the renal epithelium while claudin 7 is found associated with tight junctions of the epididymus and mouse mammary cell lines. Four types of murine mammary tumors were examined. In all claudin 7 showed a distinct cytosolic and basolateral distribution. We find claudins 1, 3, 4, 5, 7 and 8 to be present in at least one of our mouse mammary cell lines, where they are localized to the tight junction. Only claudin 1 has been found to be consistently present in mammary epithelial tight junctions. Claudin 3 is localized to the basal and lateral borders of the cells in the virgin and pregnant animals, but not observed in lactation. Claudin 8 is prominent at the tight junctions in the mammary epithelium from the lactating animal. One paper has been submitted detailing these findings and a second is in the final stages of preparation.
- Genetic Engineering and Molecular Biology
- Medicine and Medical Research