Immobilization of Enzymes in Nanoporous Host Materials: A Nanobiotechnological Approach to Decontamination and Demilitarization of Chemical and Biological Warfare Agents
Final technical rept.
DREXEL UNIV PHILADELPHIA PA DEPT OF CHEMISTRY
Pagination or Media Count:
As proposed, we have established the concept of a novel nanobiotechnology for decontamination and demilitarization of chemical and biological warfare agents, based on immobilizing decondemil effective enzymes such as organophosphorus acid anhydrolases OPAA in nanoporous host materials such as silica. The immobilization has been achieved by encapsulating the enzymes in nanoporous silicate or organically modified silicate frameworks prepared by the nonsurfactant-templated sol-gel technology recently developed in our laboratory. Such enzyme guest-nanoporous silica host systems offer many advantages over the use of free enzymes, including 1 significantly improved thermal stability 2 retain enzymatic activity in decondemil media that contain organic solvents 3 longer shelf life and higher storage stability and 4 reusability and recyclability of the enzymes. Based on our results using diisopropyl fluorophosphate DFP as the simulant for G-type agents, encapsulated OPAA not only retains its enzymatic activity to a significant extent but also exhibits much enhanced stability. Further investigation would lead to practical applications of these novel nanobiomaterials in decontamination creamslotions and as survivable bio-decontaminants under battlefield conditions.
- Chemical, Biological and Radiological Warfare
- Inorganic Chemistry
- Organic Chemistry