Genomic and Expression Profiling of Benign and Malignant Nerve Sheath Tumors in Neurofibromatosis Patients
Annual rept. 1 May 2003-30 Apr 2004
LELAND STANFORD JUNIOR UNIV STANFORD CA
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The goal of this project is to identify genes involved in the malignant transformation of neurofibromas to malignant peripheral nerve sheath tumors using expression profiling and array-based comparative genomic hybridization. The significance of the genes will be validated on much larger numbers of cases using antibodies and in situ hybridization probes on tissue microarrays TMAs. Genes will be further studied in in vitro experiments using cell lines from nerve sheath tumors. While the grant starting date was on Nay 1, 2003 authorization to work with human subjects was not obtained until April 1, 2004. Therefore this annual report will only describe the actual work performed in April 2004. Nevertheless the following progress has been made in the past year 1. The number of cases of nerve sheath tumors available will be more than sufficient to perform the first aim of this study. 2. We have started to run expression profiling and gene microarrays on a number of nerve sheath tumors and since April 1, 2004 have analyzed six malignant peripheral nerve sheath tumors and five schwannomas. 3. We have gained much experience with in situ hybridization on TMAs. This experience will enormously benefit this project.
- Genetic Engineering and Molecular Biology
- Anatomy and Physiology
- Medicine and Medical Research