Accession Number:

ADA426839

Title:

Structural Studies on Toxins and Virulence Factors of Yersinia pestis

Descriptive Note:

Annual rept. 1 Jul 2003-30 Jun 2004

Corporate Author:

BROOKHAVEN NATIONAL LAB UPTON NY

Personal Author(s):

Report Date:

2004-07-01

Pagination or Media Count:

9.0

Abstract:

In this first annual report we present our progress on two different areas, protein production and crystallization. We are working on expression and purification of Yop proteins and their complexes. In the first year we learnt reasons why some of the Yop proteins are not crystallizing. We are using a novel approach of making complexes with synthetic peptides or partner proteins to overcome this problem. In the case of LcrV, we are using the inherent property of LcrV forming a coiled coil complex with LcrG to crystallize the complex.

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research
  • Pharmacology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE