Landscape Phage Probes for Salmonella Typhimurium
AIR FORCE INST OF TECH WRIGHT-PATTERSON AFB OH SCHOOL OF ENGINEERING AND MANAGEMENT
Pagination or Media Count:
We selected from a landscape phage library probes that bind preferentially Salmonella typhimurium cells compared with other Enterobacteriaceae. The specificity of the phage probes for Salmonella typhimurium was analyzed by the phage-capture test, the enzyme-linked immunosorbent assay ELISA and the precipitation test. Interaction of representative probes with Salmonella typhimurium was characterized by fluorescence-activated cell sorting FACS, and fluorescent, optical and electron microscopy. The results show that the landscape phage library is a rich source of highly specific and robust probes for Salmonella typhimurium suitable for long-term use in continuous monitoring devices and biosorbents.