Accession Number:

ADA426566

Title:

Time-Lapse Confocal Imaging of Development of Bacillus anthracis in Macrophages

Descriptive Note:

Journal article

Corporate Author:

ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD

Report Date:

2004-03-19

Pagination or Media Count:

5.0

Abstract:

Macrophages attempt to battle infection with Bacillus anthracis spores by phagocytosis of the spores. However, it is believed that B. anthracis spores may survive phagocytosis and may actually use the macrophages that ingest them as a means of transport to lymph nodes. Thus far, the events that occur after spores undergo phagocytosis have remained unclear. To elucidate the fate of spores internalized by macrophages, we have used time-lapse confocal microscopy to follow individual fluorescent spores over time. By use of this method, we have determined that some phagocytized spores survive beyond germination, to become bacilli that then replicate within the macrophages.

Subject Categories:

  • Medicine and Medical Research
  • Toxicology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE