Accession Number:

ADA426338

Title:

Involvement of 53BP1, a p53 Binding Protein, in Chk2 Phosphorylation of p53 and DNA Damage Cell Cycle Checkpoints

Descriptive Note:

Annual summary rept. 17 Apr 2003-16 Apr 2004

Corporate Author:

BAYLOR COLL OF MEDICINE HOUSTON TX

Personal Author(s):

Report Date:

2004-05-01

Pagination or Media Count:

23.0

Abstract:

We have shown that 53BP1, a tumor suppressor p53 binding proteins, is a key transducer in the DNA damage response signaling. Inhibition of 53BP1 by siRNA in human cancer cell lines resulted in defective S-phase and G2M checkpoints in response to ionizing irradiation IR. Mouse embryonic fibroblast MEF cells generated from 53BP1 -- mice were hypersensitive to IR, and displayed a slight G2M checkpoint deficiency in response to lower dose of IR. We also showed that 53BP1 binds to p53, Chk2 and Brca1. 53BP1 plays an important role in p53 stabilization, phosphorylation of Chk2 and Brca1 in response to IR.

Subject Categories:

  • Biochemistry
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE