Toxicity of Nitro-Heterocyclic and Nitroaromatic Energetic Materials to Enchytraeid Worm.Enchytraeus Crypticus in a Natural Sandy Loam Soil
Final rept. Apr 2001-Jul 2003
EDGEWOOD CHEMICAL BIOLOGICAL CENTER ABERDEEN PROVING GROUND MD RESEARCH AND TECHNOLOGY DIR
Pagination or Media Count:
U.S. Environmental Protection Agency is developing Ecological Soil Screening Level Eco-SSL values for ecological risk assessment of contaminants at Superfind sites. Insufficient information for RDX, HMX, 2,4-DNT, 2,6-DNT, and TNB to generate Eco-SSLs necessitated standardized toxicity testing to fill the data gaps. The Enchytraeid Reproduction Test ISO163872001 was adapted using Enchytraeus crypticus in these studies. Tests were conducted in Sassafras sandy loam soil, which supports relatively high bioavailability of energetic materials. Weatheringaging procedures for amended soil were incorporated in the study to better reflect the exposure conditions in the field soils. Definitive toxicity tests conducted with both freshly amended and weatheredaged amended soils showed that EM toxicity order based on ECsub 20 values for juvenile production in tests with E. crypticus was TNB 2,4-DNT 2,6-DNT RDX with ECsub 20 values of 45, 116, 194, and 585 mg kgexp -1, respectively. The octahydro-1, 3, 5, 7 - tetranitrol, 3, 5, 7 - tetrazocine HMX did not adversely affect adult survival or juvenile production up to 21750 mg kgexp -1 treatment. These study results will be provided to the Eco-SSL workgroup for review and inclusion in the Eco-SSL database, and for developing Eco-SSLs for RDX, HMX, 2,4-DNT, 2,6-DNT, and TNB.
- Solid Wastes and Pollution and Control