Accession Number:

ADA426105

Title:

Tyronsine Kinase Gene Expression Profiling in Prostate Cancer

Descriptive Note:

Final rept. 1 Mar 2000-28 Feb 2004

Corporate Author:

CALIFORNIA UNIV BERKELEY LAWRENCE BERKELEY LAB

Personal Author(s):

Report Date:

2004-03-01

Pagination or Media Count:

15.0

Abstract:

The expression of protein kinases genes if often altered in tumors. The aberrant expression of several of these genes typically parallels the progression toward a more malignant phenotype. We developed a cDNA micro-array-based screening system to mearuser the level of expression of tyronsine kinase tk genes. The hardware for preparation of cDNA micro-arrays and basic protocols for hybridization were developed in year 1. In the second year, we finished cDNA synthesis from prostate cancer cell lines and 6 frozen tissue specimens. We continued our DNA sequencing effort and added additional targets to our micro-arrays. Using prostate cancer cell lines, the system delivered reproducible results about tk gene expression during cell transformation and progression toward a more malignant phenotype. Comparing the absolute expression levels form cDNA micro-arrays with data from Northern blot analyses suggested that our initial approach using mixed-based oligonucleotide primers led to lowered representation of highly abundant transcript. This problem has been addressed with a new primer design to be better suited to investigate the tk gene expression in small samples. We then applied our protocols to the gene expression analysis of micro-dissected tissues, and found reliable detection of abl gene overexpression with an RNA equivalent of 3 cells.

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE