Drug Development and Conservation of Biodiversity in West and Central Africa
Annual rept. 1 May 2002-30 Apr 2003
PACE UNIV NEW YORK NY
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This report concerns activities of Associated Project 3 AP3 of the International Cooperative Biodiversity Group Program ICBC directed by the Walter Reed Army Institute for Research WRAIR. This project examines medicinal plants of Central and West Africa as anti-typanosomal and anti-trichomonal agents. In the reporting period 85 plant extracts were received from WRAIR and 34 were received from the University of Dschang Cameroon, AP2. Extracts were screened in vitro vs. blood stream forms of African trypanosomes Trypanosoma brucei, Trypanosoma rhodesiense and pathogenic trichomonads Trichomonas vaginalis, Tritrichomonas foetus. In the trypanosome growth screen, 58 WRAIR extracts and 8 AP2 extracts were tested. Eight WRAIR extracts were tested vs metronidazole-resistant and -sensitive strains of T. vaginalis and another 31 were studied in a T. foetus screen. Of 58 WRAIR extracts tested 24 had IC50 values 20 microgml in the trypanosome screen. For trichomonads 2 of 8 WRAIR extracts had MIC values 0.6mgml for metronidazole-resistant T. vaginalis 4 of 31 extracts were similarly active for T. foetus. For trypanosomes, plant genera yielding active extracts included Melian, Holarrhena, Jatropha and Boerrhavia. For trichomonads, active extracts were from Aspilia, Combretum, Enantia, Hoslundia, Mormadica, Phyllanthus and Cleistophotis.