Accession Number:

ADA422943

Title:

Atomic Crystal Structure of an Organophosphorus Acid Anhydrolase

Descriptive Note:

Final progress rept. 5 Dec 2000-4 Dec 2003

Corporate Author:

BAYLOR COLL OF MEDICINE HOUSTON TX

Report Date:

2004-05-09

Pagination or Media Count:

4.0

Abstract:

The major aim is to determine the three-dimensional atomic structure of an organophosphorus acid anhydrolase OPAA by x ray crystallography. This structure is a prerequisite for remodeling the active site, in collaboration with scientists at ECBC and Geo-Centers, Inc. in order to enhance catalytic activity towards fluorinated U-type and other extremely toxic chemical warfare CW nerve agents. A decontamination system based on the remodeled OPAA not only provides rapid removal of CW agents, but is also enviromnentally safe and noncorrosive in nature. After overcoming the major difficulty in crystallizing OPAA, we have now determined by MAD and SAD techniques and refined the crystal structure of OPAA to 2.5 A resolution. The structure analysis of OPAA with the bound enzyme inhibitor MIPAFOX N,N-di-isopropylphosphorodiamidic fluoride is in progress.

Subject Categories:

  • Organic Chemistry
  • Chemical, Biological and Radiological Warfare
  • Atomic and Molecular Physics and Spectroscopy

Distribution Statement:

APPROVED FOR PUBLIC RELEASE