Process Research and Development of Antibodies as Countermeasures for C. botulinum
Annual rept. 1 Mar 2003-28 Feb 2004
NEBRASKA UNIV LINCOLN
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This report describes the project to fill out shell space on the third floor of Othmer Hall, the new home for the University of Nebraska-Lincoln Biological Process Development Facility. Construction is 90 completed and all research laboratories of the BPDF will be functional in May 2004. Detailed design of the cGMP basement has been initiated and is expected to be completed by Fall 2004. The third floor will house all of the process research capabilities, i.e. molecular biology, fermentation, cell culture, analytical methods, quality control, purification development and media prep. This report also describes research on the expression of antibodies in Chinese Hamster Ovary CHO cells against serotype A botulinum neurotoxin. We have been able to clone and successful express the full intact S-25 antibody provided by Dr. Jim Marks of UCSF at 30 mgL in 250 mL spinner shake flasks. A manuscript has been submitted on this work.
- Medicine and Medical Research