Accession Number:

ADA422920

Title:

IGF-Regulated Genes in Prostate Cancer

Descriptive Note:

Annual rept.

Corporate Author:

OREGON HEALTH SCIENCES UNIV PORTLAND

Personal Author(s):

Report Date:

2004-02-01

Pagination or Media Count:

10.0

Abstract:

We hypothesize that genes that are differentially expressed as a result of the decreased IGF-I receptor gene expression seen in metastatic prostate cancer contribute to prostate cancer progression, and include metastasis-regulating genensthat could constitute valuable diagnostic markers or therapeutic targets. We initially proposed three specific aims 1Identification of differentially expressed genes in isogenic metastatic vs. non- metastatic prostate epithelial cells 2Identification of proteins that are differentially secreted in these cell lines, and 3 Assessment of he differential expression of these genes and proteins in laser-mircrodissected samples. We have used microarray gene profiling to characterize differentially expressed genes and have used SELDI-TOF mass spectrometry to identify proteins that are differentially secreted into conditioned media. We have additionally initiated a 3 dimensional culture system to grow prostate cells in a microgravity environment that more accurately replicates in vivo cell organization and phenotype. We have discovered that elevated IGF-I receptor expression controls survival in adult human male serum, which may explain the relationship between IGF action and metastasis. We propose to take advantage of these new findings by investing the molecular mechanisms underlying this effect and the cell-surface molecules expressed in metastatic and non-metastatic cells responsible for the differential sensitivity to serum.

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE