Involvement of a Human Endogenous Retrovirus in Breast Cancer
Final rept. 1 Aug 2000-31 Jul 2003
TULANE UNIV HEALTH SCIENCE CENTER NEW ORLEANS LA
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Our overall goal is to determine whether human mammary tumor virus HMTV sequences, present as an endogenous retrovirus, are involved in a subset of human breast cancers. In studies completed for first specific aim, samples form a cohort 200 of breast cancer and appropriate control patients were obtained. Tn studies under the second specific aim, we identified and sequenced HMTV nucleic acids in breast cancer tissue, control tissue, and blood of patients form our cohorts. Results form a subset of blinded blood samples found that blood from approximately 20 of controls and 70 of breast cancer patients were positive for HMTV sequences. The third specific aim was to construct DNA and cDNA libraries from tissues positive for HNTV proviurses. As this task ahs been accomplished by others Pogo and Mason, we are focused on confirming the blinded PCR results in the entire cohort, which is nearly completed. The studies under specific aim four, to express HMTV proteins in an insect cell system, and to characterize the immunological reactions of breast cancer patients and controls against HMTV proteins, are in progress. If a definite link is established, HMTV will provide a target for vaccine development and breast cancer therapy.
- Anatomy and Physiology
- Medicine and Medical Research