Accession Number:

ADA422403

Title:

Cell Motility and Invasiveness of Neurofibromin-Deficient Neural Crest Cells and Malignant Triton Tumor Lines

Descriptive Note:

Annual rept. 1 Oct 2002-30 Sep 2003

Corporate Author:

TEXAS UNIV HEALTH SCIENCE CENTER AT SANANTONIO

Personal Author(s):

Report Date:

2003-10-01

Pagination or Media Count:

20.0

Abstract:

Our purpose is to examine the role of the NF1 gene product, neurofibromin, in modulating the migratory and invasive properties of neural crest cells NCC and neural crest-derived sarcoma cells. As a negative regulator of Ras signaling, neurofibromin may influence the responses of NC-derived cells to growth factors and extracellular matrix ECM molecules that affect motility. We use embryonic NCC and NC-derived sarcoma lines isolated from cisNf1p53 mice to compare integrin ECM receptor expression patterns, ECM adhesion preferences, migration on ECM substrata, invasion through ECM barriers, and dispersal along NCC pathways in vivo for wild-type and neurofibromin-deficient cells. In the past year, we have completed studies on the invasiveness of branchial arch mesenchymal cells and trigeminal neural crest cells isolated from Nf--, -, and mouse embryos. Consistent with published results for neurofibromin deficient astrocytes, mast cells, and Schwann cells, our data indicate that Nf1 mutant cranial neural crest cell populations are more invasive through laminin and fibronectin matrices.

Subject Categories:

  • Genetic Engineering and Molecular Biology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE