Accession Number:

ADA422009

Title:

Cellular and Molecular Changes of the Respiratory System in Rats Exposed to ACM Combustion

Descriptive Note:

Final rept. 1 Oct 2000-1 Jul 2003

Corporate Author:

NAVAL HEALTH RESEARCH CENTER SAN DIEGO CA

Personal Author(s):

Report Date:

2003-08-01

Pagination or Media Count:

32.0

Abstract:

Rats exposed to advanced composite materials ACM combustion atmospheres develop a time dependent pulmonary inflammation characterized by the influx of polymorphonuclear neutrophils PMNs into the lumen of the lung, epithelial permeability changes and inflammatory cytokine release. Mice with defective tumor necrosis factor alpha TNF-alpha gene produced significantly less interleukin-l beta IL-1beta and IL-6 while mice with defective IL-1beta gene produced significantly more TNF-alpha with on effect on IL-6. Both strains had significantly fewer BALF cells and PMNs but no effect on epithelial permeability. The use of antibodies towards TNF-alpha and IL-1beta had no effect on epithelial permeability, BALF IL-1beta or TNF-alpha. IL-1beta antibodies did not affect BALF cell numbers but did significantly increase PMNs. No effect was noted on IL-6 levels with anti-IL-1beta. TNF-alpha antibodies significantly increased the BAbF cell numbers and IL-6 but significantly decreased the percentage of PMNs in the BALF. In conclusion, gene silencing was effective in reducing the inflammation TNF-alpha IL-1beta from ACM combustion atmospheres while pretreatment with TNF-alpha or IL-1beta antibodies under the current protocol is ineffective.

Subject Categories:

  • Biochemistry
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE