Accession Number:

ADA422003

Title:

Blood-Brain Barrier Transport of Uranium

Descriptive Note:

Annual rept. 1 Sep 2002-31 Aug 2003

Corporate Author:

WAKE FOREST UNIV WINSTON-SALEM NC SCHOOL OF MEDICINE

Personal Author(s):

Report Date:

2003-09-01

Pagination or Media Count:

9.0

Abstract:

Studies in Year 2 continued according to the original plan. Studies were carried out in RBE4 cells and in primary astrocyte cultures. In both cell types, there was a significant increase in 54manganese uptake due to the addition of uranium to the media. Incubation with uranium did not significantly alter DMT-1 may not be the putative transporter for uranium across the blood-brain barrier. In addition studies, which were delayed by the lack of availability of DU pellets, we implanted rats in the gastrocnemius muscle with 10 pellets in each leg for a total of 20 pellets. The following doses were administered sham20 tantalum Ta pellets low16 Ta and 4 du medium10 Ta and 10 du high4 Ta and 16 du. The three-month cohort will be analyzed shortly for uranium brain levels, as well as DMT-1 expression in various brain regions. Rats have been monitored e.g. body weights, coat quality, and there are no noted differences in body weights in either the three-month or six-month cohorts Figure 4 or physical appearance have occurred in rats receiving surgery both Ta and du pellets compared to non-surgical controls.

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE