Accession Number:

ADA422001

Title:

Identification of a Gene on Chromosome 18q21 Involved in Suppressing Metastatic Prostate Cancer

Descriptive Note:

Annual rept. 1 Dec 2002-30 Nov 2003

Corporate Author:

TEXAS UNIV HEALTH SCIENCE CENTER AT SANANTONIO

Personal Author(s):

Report Date:

2003-12-01

Pagination or Media Count:

8.0

Abstract:

Numerous chromosomal regions have been implicated as potential locations for tumor suppressor genes involved in prostate caner including 8p, 10q, 11p, 16q, 17p, 18q, 21 and Y. In addition, in metastatic prostate cancer there is increased frequency of loss of genetic material from chromosome 18q. We have previously identified two distinct regions on chromosome 18q as the site of a tumor suppressor gene involved in metastasis suppression. In order to identify the genes on 18q involved in metastasis suppression, we have created custom bacterial artificial chromosome BAC microarrays containing genomic DNA form the two regions of 18q that are lost at a high frequency in metastatic prostate cancer. We are in the process of analyzing the data obtained form the hybridization of prostate cancer DNA to the microarray slide. We have also analyzed the expression of known genesexpressed sequence tagged sites ESTs form these town regions on 18q in normal prostate epithelium and in metastatic prostate cancer cell lines. Interestingly, two of the ESTs in the distal region exhibit altered expression when comparing less aggressive prostate cancer to highly-metastatic prostate cancer. The data generated form this proposal will pinpoint the location of the proposed metastasis suppressor gene.

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE