Development of Genetic Therapies for the Hemidesmosol Subtypes of Junction Epidermolysis Bullosa
Final rept. 1 Nov 1999-31 Oct 2003
COLUMBIA UNIV NEW YORK
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During the final award period of this project, we focused on creating the model systems for the in vitro gene therapy experiments, in particular, the in vitro skin model. We worked extensively on development of the in vitro skin model, and succeeded in generating recombinant skin between keratinocytes and dermal cells in skin equivalents. To extend these studies and make them more widely applicable for wound care enhancement in blistering diseases as well as in chemically induced wounding, we then went on to develop a model for in vitro epithelial reprogramming, in which we have begun to utilize different epithelial cell types as donor cells, in addition to keratinocytes, specifically, amnion and cornea cells. Collectively, we have shown that the use of gene delivery combined with epithelial cell-skin equivalent models, show significant promise toward developing a cellular bandage for both genetically and chemically induced skin blistering.
- Anatomy and Physiology
- Medicine and Medical Research