DID YOU KNOW? DTIC has over 3.5 million final reports on DoD funded research, development, test, and evaluation activities available to our registered users. Click
HERE to register or log in.
Accession Number:
ADA418361
Title:
Differential Roles of Insulin Receptor Substrate-1 and -2 (IRS-1, IRS-2) in Insulin-Like Growth Factor Signaling in Breast Cancer Cells
Descriptive Note:
Annual summary rept. 24 May 2002-23 May 2003
Corporate Author:
MINNESOTA UNIV MINNEAPOLIS
Report Date:
2003-06-01
Pagination or Media Count:
7.0
Abstract:
Signaling of insulin-like growth factor-I IGF-I through the type I insulin-like growth factor receptor IGF-IR has been shown to regulate breast cancer cell proliferation, survival and metastasis in vitro. Recent evidence indicates insulin receptor substrate-1 and -2 IRS-1 and IRS-2, the primary signaling molecules utilized by the IGF-IR, may mediate distinct IGF-I effects. To investigate the specific functional roles of the IRS species in mediating IGF action, we utilized the T47D-Y and T47D-YA breast cancer cell lines, which lack both IRS-1 and IRS-2 expression yet express a functional IGF-IR, to generate cell lines that independently express IRS-1 or IRS-2. T47D-Y and T47D-YA breast cancer cells were stably transfected with either human IRS-1 or IRS-2 cDNA, screened for IRS expression and activation by immunoblotting, and analyzed for IGF responsiveness in proliferation and motility assays.
Distribution Statement:
APPROVED FOR PUBLIC RELEASE