Neurotoxicity From Chronic Exposure to Depleted Uranium
Annual rept. 1 Apr 2002-31 Mar 2003
ILLINOIS UNIV AT CHICAGO
Pagination or Media Count:
This project is designed to test the hypothesis that chronic exposure to depleted uranium DU impairs neuronal processes underlying cognitive function via alterations induced at hippocampal glutamatergic synapses. As prescribed by the Statement of Work, efforts were initiated in year I on Technical Objectives 1 establishment of chronic exposure protocol and 2 defining integrity of hippocampal glutamate release and are continuing into year 2. Blood and brain tissue are being collected in three exposure groups 0, 300, and 600 mg implanted DU pellets across periods of 1-12 months in duration, and will be analyzed in two sample cohorts in year 2. A superfusion procedure has been successfully merged with liquid chromatography to measure stimulated hippocampal synaptosomal glutamate and GABA release in the presence and absence of Ca2 see Figures 1-2, and these experiments have begun utilizing acute exposure to uranyl ion. In addition, the component of Technical Objective 2 involving chronic exposure studies has been initiated by implantation of DU pellets in the majority of animals to be tested by intracerebral microdialysis. Thus, despite significant obstacles to progress the project is proceeding according to the Statement of Work schedule.
- Medicine and Medical Research
- Inorganic Chemistry