Signal Transduction by Designed Metal-Binding Proteins
Final rept. 1 May 1996-30 Apr 1999
YALE UNIV NEW HAVEN CT DEPT OF MOLECULAR BIOPHYSICS AND BIOCHEMISTRY
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The objective of this research is to develop designed metal-binding proteins to report upon the presence of metal ions in solution. We use small, robust protein frameworks as scaffolds on which to design novel metal-ion binding sites. The metal binding site design is computational, to allow an exhaustive sampling of all possible sites. The sites are designed with defined geometries and a variety of primary ligands to metal, which allow a range of different metal-ion binding specificities and affinities to be arrayed. The aim is to couple the metal-binding event to a change in fluorescence of an appropriate probe. For practical applications, reagent-less systems are preferred, but in the development stages, extrinsic reporter probes are also investigated.
- Organic Chemistry