Accession Number:

ADA416956

Title:

Signal Transduction by Designed Metal-Binding Proteins

Descriptive Note:

Final rept. 1 May 1996-30 Apr 1999

Corporate Author:

YALE UNIV NEW HAVEN CT DEPT OF MOLECULAR BIOPHYSICS AND BIOCHEMISTRY

Personal Author(s):

Report Date:

2003-08-05

Pagination or Media Count:

3.0

Abstract:

The objective of this research is to develop designed metal-binding proteins to report upon the presence of metal ions in solution. We use small, robust protein frameworks as scaffolds on which to design novel metal-ion binding sites. The metal binding site design is computational, to allow an exhaustive sampling of all possible sites. The sites are designed with defined geometries and a variety of primary ligands to metal, which allow a range of different metal-ion binding specificities and affinities to be arrayed. The aim is to couple the metal-binding event to a change in fluorescence of an appropriate probe. For practical applications, reagent-less systems are preferred, but in the development stages, extrinsic reporter probes are also investigated.

Subject Categories:

  • Biochemistry
  • Organic Chemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE