Pleiotrophin Signaling Through PTNR in Breast Cancer
Annual summary 1 Apr 2000-31 Mar 2003
GEORGE WASHINGTON UNIV MEDICAL CENTER WASHINGTON DC
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Angiogenesis, formation of new blood vessels, plays a key role in breast cancer growth by providing a method for hematogenous spread of the tumor, as well as providing nutrients for tumor metastasis. Rational treatment strategies for breast cancer must take into account the molecular mechanisms by which cancer develops, maintain its growth and finally spread to other parts of the body. The pleiotrophin PTN signaling pathway is known to be important in angiogenesis and breast cancer growth, but the exact mechanism by which PTN acts has not been elucidated. Recently, we identified a cell-surface receptor tyrosine kinase, anaplastic lymphoma kinase ALK receptor, as a receptor for PTN. In this report, due to lack of a good ALK antibody, generation of a tagged full-length ALK was created to facilitate the detection of ALK. Utilization of a tagged ALK-ECD construct showed that it co-immunoprecipitated with PTN. Although the precise region of PTN binding site is still unknown due to unsuccessful attempts at creating ALKdelta368-406, success at creating other ALK mutants showed that the site-directed mutagenesis could be employed to create ALK mutants.
- Genetic Engineering and Molecular Biology
- Anatomy and Physiology
- Medicine and Medical Research